COMPARATIVE CYTOGENETIC STUDIES ON SPONTANEOUS AND INDUCED ABNORMALITIES OF MOUSE EMBRYOS
Ovulation was induced in inbred and random bred mice. From day 6 to day 15 of gestation pregnant mice were sacrificed and their litters examined. Morphological abnormalities were noted. Chromosome analyses were also performed using the trypsin-Giemsa (G) and Quinacrine mustard (Q) banding techniques. The study showed an increased occurrence of chromosomal abnormalities in the embryos of superovulated mice as compared to the embryos of spontaneously ovulated mice. Monosomies, trisomies and mosaics were the most common types of chromosomal abnormalities observed. Post-implantation embryonic death was also found to be higher among the embryos of superovulated mice. Day 6 of gestation was shown to be the earliest time at which a site of resorption could be detected. This study also dealt with the effects of actinomycin-D on the F(,2) embryos of mice treated at various stages of gestation (day 0 to day 5). Treated inbred mice were allowed to continue pregnancy to normal parturition. The litters of these mice (F(,1)) were nursed and weaned as usual. Sexually mature F(,1) females were allowed to mate with normal (untreated) males. From day 6 to day 15 of gestation, pregnant F(,1) mice were sacrificed and their litters examined. As with the superovulation studies, any morphological abnormalities were noted and chromosome analyses were performed. The study did not indicate an increase in the number of chromosomal abnormalities or higher post-implantation embryonic mortality in the F(,2) embryos.
LUCKETT, DEBORAH CECILE, "COMPARATIVE CYTOGENETIC STUDIES ON SPONTANEOUS AND INDUCED ABNORMALITIES OF MOUSE EMBRYOS" (1981). ETD Collection for Fordham University. AAI8123557.