Document Type
Article
Disciplines
Biochemistry
Abstract
Glutamate dehydrogenase (GDH) activity was determined in high-speed fractions (1 00,000 g for 60 min) obtained from whole rat brain homogenates after removal of a low-speed pellet (480 g for 10 min). Approximately 60% of the high-speed GDH activity was particulate (associated with membrane) and the remaining was soluble (probably of mitochondrial matrix origin). Most of the particulate GDH activity resisted extraction by several commonly used detergents, high concentration of salt, and sonication; however, it was largely extractable with the cationic detergent cetyltrimethylammonium bromide (CTAB) in hypotonic buffer solution. The two GDH activities were purified using a combination of hydrophobic interaction, ion exchange, and hydroxyapatite chromatography. Throughout these purification steps the two activities showed similar behavior. Kinetic studies indicated similar Km values for the two GDH fractions for the substrates a-ketoglutarate, ammonia, and glutamate; however, there were small but significant differences in Km values for NADH and NADPH. Although the allosteric stimulation by ADP and L-leucine and inhibition by diethylstilbestrol was comparable, the two GDH components differed significantly in their susceptibility to GTP inhibition in the presence of 1 mM ADP, with apparent Ki values of 18.5 and 9.0 ~M GTP for the soluble and particulate fractions, respectively. The Hill plot coefficient, binding constant, and cooperativity index for the GTP inhibition were also significantly different, indicating that the two GDH activities differ in their allosteric sites. In addition, enzyme activities of the two purified proteins exhibited a significant difference in thermal stability when inactivated at 45°C and pH 7.4 in 50 mM phosphate buffer. Key Words: Glutamate dehydrogenase-Particulate- Soluble-GTP-Allosteric regulation. Colon A. D. et al. Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain. J. Neurochem. 46, 1811-1819 (1986)
Article Number
1021
Publication Date
1986
Recommended Citation
Colon, Arlene D.; Plaitakis, Andreas; Perakis, Antonis; and Clarke, Donald Dudley PhD, "Purification and characterization of a soluble and a particulate glutamate dehydrogenase from rat brain / Arlene D. Colon, Andreas Plaitakis, Antonis Perakis, Soll Berl, and Donald D. Clarke Department of Neurology, Mount Sinai School of Medicine, New York, and Department of Chemistry, Fordham University, Bronx, New York, U.S.A." (1986). Chemistry Faculty Publications. 22.
https://research.library.fordham.edu/chem_facultypubs/22
Comments
Journal of neurochemistry pages 1811-1819. http://dx.doi.org/10.1111/j.1471-4159.1986.tb08500.x