Document Type
Article
Disciplines
Biochemistry
Abstract
In experiments reported some years ago the enzyme-catalyzed exchange of the amide group of glutamine and asparagine with 15N ammonia was demonstrated but an exchange of the amide groups of protein bound dicarboxylic amino acids could not be explored owing to the unfavorable experimental conditions1 . Recently the problem of the metabolism of protein-amide groups was approached again with the aid of 14C-labeled amines, such as ethanolamine or cadaverine. Soluble protein fractions obtained from sucrose homogenates (o.2sM) of the livers of guinea pigs, rats, mice, and rabbits by centrifugation at roo,ooo X g were incubated in the presence of Ca++ with the 14C-labeled amines. Appreciable radioactivity was recovered in the proteins precipitated and purified by the method of SIEKEVITZ2 (Table I). The incorporating system was activated by Ca++ but not by Mn++- and Mg++ or other metal ions-a fact which makes it unlikely that we are dealing with the enzyme system responsible for glutamo or asparto-transferase activities
Article Number
1039
Publication Date
1957
Recommended Citation
Sarkar, Nirmal K.; Clarke, Donald Dudley PhD; and Waelsch, Heinrich, "An enzymically catalyzed incorporation of amines into proteins / Nirmal K. Sarkar Donald D. Clarke Heinrich Waelsch Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, and the New York Psychiatric Institute, New York, N.Y. (U.S.A.)" (1957). Chemistry Faculty Publications. 40.
https://research.library.fordham.edu/chem_facultypubs/40
Comments
Biochim. biophys. acta 25:451-452. http://dx.doi.org/10.1016/0006-3002(57)90512-7