A relationship of n-acetylaspartate biosynthesis to neuronal protein synthesis / D. D. Clarke, S. Greenfield, E. Dicker, L.J. Tirri, and E.J. Ronan Chemistry Department, Fordham University, Bronx, NY 10458, U.S.A.

Authors

Donald Dudley Clarke PhD, Fordham UniversityFollow
S. Greenfield, Aarhus universitet
E. Dicker, Fordham University
L. J. Tirri, Albert Einstein College of Medicine

Document Type

Article

Disciplines

Biochemistry

Abstract

A detailed time study of the incorporation of label from sodium-[1-14C]acetate, [1-14C]ethanol, and [2-14C]glucose into the aspartyl moiety of N-acetylaspartic acid (NAA) was conducted. As expected the specific activity of aspartate increased rapidly with time and peaked within 15-20 min after which it fell sharply: but significantly that of the aspartyl moiety of NAA rose very slowly even after the specific activity of aspartate had fallen to less than 1 per cent of the peak values. A rat brain microsomal free supernatant preparation was shown enzymatically to incorporate label from sodium-[l-14C]acetate into the t-RNA fraction from which was isolated N-[1-14C]acetylaspartic acid. From these observations we were inclined to speculate that NAA-t-RNA may serve as an initiator of neuronal protein synthesis

Article Number

1068

Publication Date

1975

Recommended Citation

Clarke, Donald Dudley PhD; Greenfield, S.; Dicker, E.; and Tirri, L. J., "A relationship of n-acetylaspartate biosynthesis to neuronal protein synthesis / D. D. Clarke, S. Greenfield, E. Dicker, L.J. Tirri, and E.J. Ronan Chemistry Department, Fordham University, Bronx, NY 10458, U.S.A." (1975). Chemistry Faculty Publications. 69.
https://research.library.fordham.edu/chem_facultypubs/69

Comments

Journal of neurochemistry 24:479-485. http://dx.doi.org/10.1111/j.1471-4159.1975.tb07665.x